Product Datasheet

 
Product Description
 
Product: Immobilized human antithrombin
Catalog Number: HATIII-I
Product Page: https://www.mol-innov.com/products/IHUATIIIIMM1ML
Description: May be used repeatedly. Protocol for purifying high affinity heparin with immobilized antithrombin:
1. Equilibrate immobilized antithrombin in TBS (0.1M Tris-HCl, 0.15M NaCl, pH 7.4) or PBS (0.05M Sodium Phosphate, 0.15M NaCl, pH 7.4).
2. Apply heparin in TBS or PBS. Binding capacity is ~0.1 mg high affinity heparin / ml resin and must be determined by the end user.
3. Wash and elute heparin with 3M NaCl in TBS or PBS.
4. Re-equilabrate resin in TBS or PBS.
5. Add 0.02percent Sodium Azide for storage.
Recommended Storage: SAMPLE
Minimum Shelf Life: 210.00
Source: 1.0 mg/ml coupled
Molecular Weight: Proteins
Extinction Coefficient: 58,200
Antigen: 4 C
Target: Resin
Target Molecular Weight: IMMOBILIZED PROTEINS
Host: 1865.5
Clonality: 30
Isotype: Human plasma
Applications: Cold pack
Cross-reactivity: 12 months from delivery
 
Sample Characteristics
 
Lot Number: HATIII-I-IHUATIIIIMM10ML
Aliquot: 1 x Human Antithrombin III Immobilized 10ml
Concentration: 300
Volume: 2665
Specific activity: SodiumAzide
Titer: 0.1M Tris-HCl; 0.15M NaCl; 0.02% Sodium Azide; pH 7.4
Buffer: SAMPLE
Form: 1.0 ml
Preservative: 2.0 ml of 1:1 slurry
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com