Product Datasheet
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Product Description |
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Product: |
Immobilized human antithrombin |
Catalog Number: |
HATIII-I |
Product Page: |
https://www.mol-innov.com/products/IHUATIIIIMM1ML |
Description: |
May be used repeatedly. Protocol for purifying high affinity heparin with immobilized antithrombin:
1. Equilibrate immobilized antithrombin in TBS (0.1M Tris-HCl, 0.15M NaCl, pH 7.4) or PBS (0.05M Sodium Phosphate, 0.15M NaCl, pH 7.4).
2. Apply heparin in TBS or PBS. Binding capacity is ~0.1 mg high affinity heparin / ml resin and must be determined by the end user.
3. Wash and elute heparin with 3M NaCl in TBS or PBS.
4. Re-equilabrate resin in TBS or PBS.
5. Add 0.02percent Sodium Azide for storage. |
Recommended Storage: |
SAMPLE |
Minimum Shelf Life: |
210.00 |
Source: |
1.0 mg/ml coupled |
Molecular Weight: |
Proteins |
Extinction Coefficient: |
58,200 |
Antigen: |
4 C |
Target: |
Resin |
Target Molecular Weight: |
IMMOBILIZED PROTEINS |
Host: |
1865.5 |
Clonality: |
30 |
Isotype: |
Human plasma |
Applications: |
Cold pack |
Cross-reactivity: |
12 months from delivery |
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Sample Characteristics |
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Lot Number: |
HATIII-I-IHUATIIIIMM10ML |
Aliquot: |
1 x Human Antithrombin III Immobilized 10ml |
Concentration: |
300 |
Volume: |
2665 |
Specific activity: |
SodiumAzide |
Titer: |
0.1M Tris-HCl; 0.15M NaCl; 0.02% Sodium Azide; pH 7.4 |
Buffer: |
SAMPLE |
Form: |
1.0 ml |
Preservative: |
2.0 ml of 1:1 slurry |
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Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |