Product Datasheet
|
|
|
Product Description |
|
Product: |
PAI-1 mutant - stable, no LRP binding |
Catalog Number: |
HPAI-R76E-I91L |
Product Page: |
https://www.mol-innov.com/products/IHUPAI1RLRP500UG |
Description: |
A substitution of Glutamic Acid for Arginine at position 76 greatly decreases the binding of this Human PAI-1 mutant to the low density lipoprotein receptor-related protein (LRP). The putative LRP binding exosite is thought to overlap with the heparin binding site on the PAI-1 molecule. Binding to LRP or similar receptors leads to the clearance of PAI-1 complexes. An additional mutation (Isoleucine 91 to Leucine) provides increased stability for use in long term binding experiments or in vivo studies. A human PAI-1 point mutation stable form (Catalog Number HPAI-I91L) is available as an LRP binding control PAI-1.
References: Stefansson S. et al. (1998) J Biol Chem 273:6358-6366. |
Recommended Storage: |
SAMPLE |
Minimum Shelf Life: |
500.50 |
Source: |
2.5 mg/ml |
Molecular Weight: |
Proteins |
Extinction Coefficient: |
43,000 |
Antigen: |
-70 C |
Target: |
Frozen liquid |
Target Molecular Weight: |
PAI-1 |
Host: |
819 |
Clonality: |
30 |
Isotype: |
E. coli |
Applications: |
Dry ice |
Cross-reactivity: |
3 years from delivery |
|
Sample Characteristics |
|
Lot Number: |
HPAI-R76E-I91L-IHUPAI1RLRP1MG |
Aliquot: |
1 x Human PAI-1 Recombinant LRP Binding Negative 1 mg |
Concentration: |
715 |
Volume: |
1170 |
Titer: |
0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 |
Buffer: |
SAMPLE |
Form: |
0.5 mg |
Preservative: |
0.2 ml |
|
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
|
For in vitro laboratory use only |
|
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |