Product Datasheet

 
Product Description
 
Product: PAI-1 mutant - stable, no LRP binding
Catalog Number: HPAI-R76E-I91L
Product Page: https://www.mol-innov.com/products/IHUPAI1RLRP500UG
Description: A substitution of Glutamic Acid for Arginine at position 76 greatly decreases the binding of this Human PAI-1 mutant to the low density lipoprotein receptor-related protein (LRP). The putative LRP binding exosite is thought to overlap with the heparin binding site on the PAI-1 molecule. Binding to LRP or similar receptors leads to the clearance of PAI-1 complexes. An additional mutation (Isoleucine 91 to Leucine) provides increased stability for use in long term binding experiments or in vivo studies. A human PAI-1 point mutation stable form (Catalog Number HPAI-I91L) is available as an LRP binding control PAI-1.

References:
Stefansson S. et al. (1998) J Biol Chem 273:6358-6366.
Recommended Storage: SAMPLE
Minimum Shelf Life: 500.50
Source: 2.5 mg/ml
Molecular Weight: Proteins
Extinction Coefficient: 43,000
Antigen: -70 C
Target: Frozen liquid
Target Molecular Weight: PAI-1
Host: 819
Clonality: 30
Isotype: E. coli
Applications: Dry ice
Cross-reactivity: 3 years from delivery
 
Sample Characteristics
 
Lot Number: HPAI-R76E-I91L-IHUPAI1RLRP1MG
Aliquot: 1 x Human PAI-1 Recombinant LRP Binding Negative 1 mg
Concentration: 715
Volume: 1170
Titer: 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6
Buffer: SAMPLE
Form: 0.5 mg
Preservative: 0.2 ml
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
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Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com