Product Datasheet

 
Product Description
 
Product: Human Albumin ELISA Kit
Catalog Number: HSAKT
Product Page: https://www.mol-innov.com/products/IHUALBKT1KIT
Description: Albumin is a water-soluble protein with considerable structural stability which makes up 60 percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. The sensitive quantitative measurement of total human albumin (human serum albumin, HSA) in plasma, serum, urine or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. Albumin is present in normal human plasma at a concentration of 35-50 mg/ml. The assay measures human albumin in the 1-500 ng/ml range. Samples giving human albumin levels above 500ng/ml should be diluted in 1X diluent before use. A 1:1,000,000 to 1:2,000,000 dilution for normal plasma or a 1:500 to 1:1,000 dilution for urine is suggested for best results. Human albumin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-human albumin primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of human albumin in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human albumin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Also available: High quality human albumin and human prealbumin.
Recommended Storage: SAMPLE
Minimum Shelf Life: 252.00
 
Technical Information
 
Purity: Human
Molecular Weight: ELISA kits
Antigen: 4 C
Target: Complete kit
Target Molecular Weight: HUMAN PLASMA PROTEINS
Host: 1211
Clonality: 30
Isotype: Multiple
Clone: s
Applications: Cold pack
Cross-reactivity: 12 months from manufacture, see label for expiration date
Concentration: 360
Volume: 1730
Buffer: SAMPLE
Form: 1 kit
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com