Product Datasheet

Product Description
Product: Mouse CRP ELISA Kit
Catalog Number: MCRPKT
Product Page:
Description: C-reactive protein (CRP) is an acute phase reactant which is elevated in plasma in response to increased interleukin-6 induced by inflammation, infection and tissue injury. CRP is expressed mainly in the liver and activates the complement pathway following calcium-dependent binding to phosphocholine on apoptotic, necrotic and microbial cells. The sensitive quantitative measurement of total mouse CRP antigen in plasma and other biological fluids is easily performed with this 96 well strip format ELISA kit. This kit has been validated for measurement of CRP in C57 mouse plasma (20 ug/ml), Balb/C mouse plasma (3.5 ug/ml), and CD1 mouse plasma (7.2 mg/ml). The assay measures CRP antigen in the 0.02-2 ng/ml range. Samples giving mouse CRP levels above 2 ng/ml should be diluted in blocking buffer before use. For best results, dilute plasma samples 1:25,000 to 1:50,000. Mouse CRP will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse CRP primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of CRP in the samples. A standard calibration curve is prepared using dilutions of purified CRP and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Recommended Storage: 4 C
Shipping Conditions: Cold pack
Minimum Shelf Life: 12 months from manufacture, see label for expiration date
Source: Multiple
Target: C-Reactive Protein
Host: Mouse
Sample Characteristics
Aliquot: 1 x 1 kit
Form: Complete kit
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
For in vitro laboratory use only
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 -