Product Datasheet

 
Product Description
 
Product: Mouse IL-17A total antigen assay ELISA kit
Catalog Number: MIL17AKT
Product Page: http://www.mol-innov.com/products/mouse-il-17a-total-antigen-assay-elisa-kit
Description: Mouse Interleukin-17A (aka IL-17, IL-17A or CTLA-8) is a 133 amino acid disulfide-linked homodimeric glycoprotein that is the founding member of the IL-17 family of proteins. IL-17A is a proinflammatory cytokine that participates in neutrophil recruitment and is primarily expressed in CD4+ T cells. IL-17A has been shown in a mouse knockout model to play a vital role in allergen-specific immune responses via T cell activation. The sensitive quantitative measurement of total mouse IL-17A antigen in cell culture media samples is easily performed with this 96 well strip format ELISA kit. The assay measures total mouse IL-17A in the 0.01-10 ng/ml range. Samples giving mouse IL-17A levels above 10 ng/ml should be diluted in culture media or blocking buffer before use. Mouse IL-17A will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated anti-mouse IL-17A primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of IL-17A in the samples. A standard calibration curve is prepared using dilutions of purified IL-17A and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.


Recommended Storage: 4 C
Shipping Conditions: Cold pack
Minimum Shelf Life: 12 months from manufacture, see label for expiration date
Source: Multiple
Target: Interleukin 17A
Host: Mouse
 
Sample Characteristics
 
Lot Number: MIL17AKT-SAMPLE
Aliquot: 1 x 1 kit
Form: Complete kit
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com