Product Datasheet

 
Product Description
 
Product: Mouse PAI-1 genetically deficient lung
Catalog Number: MPAI-KO-LG
Product Page: https://www.mol-innov.com/products/IMSPAI1GDLG11LOBE
Description: This organ is an ideal negative control for western blotting, immunoprecipitation, immunohistochemistry and other experiments involving PAI-1. Collected and flash frozen from homozygous PAI-1 knockout mice of strain B6.129S2-Serpine1 tm1Mlg /J. Each mouse lung consists of 5 lobes. The targeted PAI-1 knockout mutation was made on the C57BL/6J background strain. Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.

References:
Carmeliet P; Kieckens L; Schoonjans L; Ream B; van Nuffelen A; Prendergast G; Cole M; Bronson R; Collen D; Mulligan RC. 1993. Plasminogen activator inhibitor-1 gene-deficient mice. I. Generation by homologous recombination and characterization. J Clin Invest 92(6):2746-55.
Recommended Storage: SAMPLE
Minimum Shelf Life: 70.00
Molecular Weight: Proteins
Antigen: -70 C
Target: Frozen tissue
Target Molecular Weight: PAI-1 KNOCKOUT MOUSE PLASMA AND ORGANS
Host: 133
Clonality: 30
Isotype: PAI-1 knockout mouse
Applications: Dry ice
Cross-reactivity: 3 years from delivery
Concentration: 100
Volume: 190
Buffer: SAMPLE
Form: 1 Lobe
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
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