Product Datasheet

 
Product Description
 
Product: Mouse PAI-1 total antigen assay ELISA kit
Catalog Number: MPAIKT-TOT
Product Page: http://www.mol-innov.com/products/mouse-pai-1-total-antigen-assay-elisa-kit
Description: Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of total mouse PAI-1 antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration level of PAI-1 antigen in mouse plasma was found to be 1.9 ng/ml. The assay measures mouse PAI-1 in the 0.05-50 ng/ml range. Samples giving mouse PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. Normal plasma should be applied directly to the plate for best results. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Mouse PAI-1 will bind to the capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will be detected by the assay. Cross-reactivity: 2percent porcine PAI-1, 1.7percent human PAI-1, 0.5percent rabbit PAI-1, 0.3percent rat PAI-1. After appropriate washing steps, anti-mouse PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse PAI-1 in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Negative control: PAI-1 genetically deficient mouse plasma
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Mouse PAI-1 Antigen Capture Plate, Secondary Antibody
Recommended Storage: 4 C
Shipping Conditions: Cold pack
Minimum Shelf Life: 12 months from manufacture, see label for expiration date
Source: Multiple
Target: PAI-1
Host: Mouse
 
Sample Characteristics
 
Lot Number: MPAIKT-TOT-SAMPLE
Aliquot: 1 x 1 kit
Form: Complete kit
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA - Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com