Product Datasheet

 
Product Description
 
Product: Mouse vitronectin total antigen assay ELISA kit
Catalog Number: MVNKT-TOT
Product Page: https://www.mol-innov.com/products/IMSVTNKTT1KIT
Description: Vitronectin is an abundant plasma glycoprotein that helps regulate coagulation, fibrinolysis, complement activation, and cell adhesion. Vitronectin binds to glycosaminoglycans, collagen, plasminogen and urokinase receptors. It also may control the clearance of vascular thrombi by binding and stablilizing PAI-1. In binding PAI-1, it extends the lifetime of active PAI-1. Vitronectin may also be involved in the regulation of bone metabolism. The sensitive quantitative measurement of total mouse vitronectin antigen in plasma, serum, culture media or tissue extract samples is easily performed with this 96 well strip format ELISA kit. The vitronectin concentration in mouse serum has been estimated at 300 ug/ml by semi-quantitative immunoblotting. The assay measures total vitronectin in the 0.1-100 ng/ml range. Samples giving mouse vitronectin levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:10,00 to 1:40,000 dilution for mouse plasma or serum is suggested for best results. Mouse vitronectin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse vitronectin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse vitronectin in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse vitronectin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Mouse Vitronectin Antigen Capture Plate, Avidin-HRP
Recommended Storage: SAMPLE
Minimum Shelf Life: 332.50
 
Technical Information
 
Purity: Mouse
Molecular Weight: ELISA kits
Antigen: 4 C
Target: Complete kit
Target Molecular Weight: VITRONECTIN ELISA KITS
Host: 1410.5
Clonality: 30
Isotype: Multiple
Clone: s
Applications: Cold pack
Cross-reactivity: 12 months from manufacture, see label for expiration date
Concentration: 475
Volume: 2015
Buffer: SAMPLE
Form: 1 kit
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com