Product Datasheet
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| Product Description |
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| Product: |
Human PAI-1 (N-terminal cysteine stable mutant) |
| Catalog Number: |
NTCYSCPAI |
| Product Page: |
https://www.mol-innov.com/products/IHUPAI1RSMNT500UG |
| Description: |
This stable form of PAI-1 contains an N-terminal cysteine mutation in addition to the four stabilization mutations (patent pending). The protein is supplied in buffer with 1mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis. |
| Recommended Storage: |
SAMPLE |
| Minimum Shelf Life: |
500.50 |
| Source: |
2.5 mg/ml |
| Molecular Weight: |
Proteins |
| Extinction Coefficient: |
43,000 |
| Antigen: |
-70 C |
| Target: |
Frozen liquid |
| Target Molecular Weight: |
PAI-1 |
| Host: |
819 |
| Clonality: |
30 |
| Isotype: |
E. coli |
| Applications: |
Dry ice |
| Cross-reactivity: |
3 years from delivery |
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| Sample Characteristics |
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| Lot Number: |
NTCYSCPAI-IHUPAI1RSMNT1MG |
| Aliquot: |
1 x Human PAI-1 Recombinant Stable Mutant N Terminal Mutation 1 mg |
| Concentration: |
715 |
| Volume: |
1170 |
| Titer: |
0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; 1mM DTT; pH 6.6 |
| Buffer: |
SAMPLE |
| Form: |
0.5 mg |
| Preservative: |
0.2 ml |
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| Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
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| For in vitro laboratory use only |
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Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |