Product Datasheet

 
Product Description
 
Product: Human PAI-1 (N-terminal cysteine, active fraction)
Catalog Number: NTCYSPAI-A
Product Page: https://www.mol-innov.com/products/IHUPAI1ARNT500UG
Description: Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The active fraction is >95percent pure and >95percent active as determined by titration with HWM tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
Recommended Storage: SAMPLE
Minimum Shelf Life: 500.50
Source: 2.5 mg/ml
Molecular Weight: Proteins
Extinction Coefficient: 43,000
Antigen: -70 C
Target: Frozen liquid
Target Molecular Weight: PAI-1
Host: 819
Clonality: 30
Isotype: E. coli
Applications: Dry ice
Cross-reactivity: 3 years from delivery
 
Sample Characteristics
 
Lot Number: NTCYSPAI-A-IHUPAI1ARNT1MG
Aliquot: 1 x Human PAI-1 Recombinant Active N Terminal Mutation 1 mg
Concentration: 715
Volume: 1170
Titer: 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; 1mM DTT; pH 6.6
Buffer: SAMPLE
Form: 0.5 mg
Preservative: 0.2 ml
 
Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
 
For in vitro laboratory use only
 
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com