Product Datasheet
|
|
| |
| Product Description |
| |
| Product: |
Human PAI-1 (N-terminal cysteine mutant, latent fraction) |
| Catalog Number: |
NTCYSPAI-L |
| Product Page: |
https://www.mol-innov.com/products/IHUPAI1RLNT500UG |
| Description: |
Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The latent fraction is <0.4percent active as determined by titration with HMW tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis. |
| Recommended Storage: |
SAMPLE |
| Minimum Shelf Life: |
500.50 |
| Source: |
2.5 mg/ml |
| Molecular Weight: |
Proteins |
| Extinction Coefficient: |
43,000 |
| Antigen: |
-70 C |
| Target: |
Frozen liquid |
| Target Molecular Weight: |
PAI-1 |
| Host: |
819 |
| Clonality: |
30 |
| Isotype: |
E. coli |
| Applications: |
Dry ice |
| Cross-reactivity: |
3 years from delivery |
| |
| Sample Characteristics |
| |
| Lot Number: |
NTCYSPAI-L-IHUPAI1RLNT1MG |
| Aliquot: |
1 x Human PAI-1 Recombinant Latent N Terminal Mutation 1 mg |
| Concentration: |
715 |
| Volume: |
1170 |
| Titer: |
0.1M HEPES; 0.1M NaCl; 1mM DTT; pH 7.4 |
| Buffer: |
SAMPLE |
| Form: |
0.5 mg |
| Preservative: |
0.2 ml |
| |
| Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet. |
| |
| For in vitro laboratory use only |
| |
Molecular Innovations, Inc. - 46430 Peary Court, Novi, MI 48377, USA
Tel: (888) 557-5055 - Fax: (248) 896-0148 - info@mol-innov.com |