The sensitive measurement of functionally active human alpha2-antiplasmin is easily performed with this 96 well strip format ELISA kit. Functionally active antiplasmin present in citrated plasma, serum, culture media or tissue extracts reacts with plasmin coated onto a micro titer plate. Complexed antiplasmin will not bind to the plate and will not be detected. After appropriate washing steps, anti human alpha2-antiplasmin primary antibody binds to the antiplasmin. Excess antibody is washed away, and bound antibody, which is proportional to the original active antiplasmin present in the plasma sample, is then reacted with the secondary antibody conjugated to HRP. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared using dilutions of purified antiplasmin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Gene Name: SERPINF2
Gene ID: 5345
Swiss-Prot/UniProt ID: P08697