The sensitive measurement of functionally active human tPA is easily performed with this 96 well strip format ELISA kit. Active tPA will bind to biotinylated functionally active PAI-1 bound to the avidin coated microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. An HRP detection system using TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples.
Cross-reactivity: 3percent Mouse TPA, 30percent Rat TPA.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody
Gene Name: PLAT
Gene ID: 5327
Swiss-Prot/UniProt ID: P00750