The sensitive measurement of functionally active mouse PAI-1 is easily performed with this 96 well strip format ELISA kit. Functionally active PAI-1 present in plasma or culture media reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, anti mouse PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away, and bound antibody, which is proportional to the original active PAI-1 present in the plasma sample, is then reacted with a secondary antibody. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Cross-reactivity: 29percent Rat PAI, 18percent Rabbit PAI, 0.6percent Porcine PAI, 76percent Human PAI.

Negative control: PAI-1 genetically deficient mouse plasma
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Plate, Secondary Antibody
Gene Name: SERPINE1
Gene ID: 18787
Swiss-Prot/UniProt ID: P22777


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