NOTE: This kit has been reformulated and is now sensitive enough to measure active tPA in normal plasma of mouse strains NSA/CF-1 (9.9 ng/ml), C57BL6 (1.4 ng/ml), and CD-1 (0.4 ng/ml).

The sensitive measurement of functionally active mouse tPA is easily performed with this 96 well strip format ELISA kit. Active tPA will bind to biotinylated functionally active PAI-1 bound to the avidin coated microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. An HRP detection system using TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples.

The assay measures active tPA in the 0.05-50 ng/ml range.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody
Gene Name: PLAT
Gene ID: 18791
Swiss-Prot/UniProt ID: P11214


Citations:
1. Norris, E.H. et al. (2007) PNAS 104: 13473-13478.
2. Roelofs, J.T.H. et al. (2006) J. Am. Soc. Nephrol. 17: 131-140.