The sensitive measurement of functionally active rat PAI-1 is easily performed with this 96 well strip format ELISA kit. Functionally active PAI-1 present in plasma or culture media reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, anti rat PAI-1 primary antibody binds to the PAI-1. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Cross-reactivity: 12percent Porcine PAI, 100percent Mouse PAI.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Plate, Secondary Antibody
Gene Name: SERPINE1
Gene ID: 24617
Swiss-Prot/UniProt ID: P20961


Citations:
1. Huang, Y et al. (2009) Am. J. Physiol. Renal Physiol. 297: F1045-F1054.
2. Deng, X. et al. (2008) J. Pharmacol. Exp. Ther. 326: 144-152.
3. Bergheim, I. et al. (2006) J. Pharmacol. Exp. Ther. 316:1053-1061.
4. Deng, X. et al. (2007) J. Pharmacol. Exp. Ther. 322:852-861.