The sensitive measurement of functionally active rat uPA is easily performed with this 96 well strip format ELISA kit. Functionally active uPA present in citrated plasma, serum, culture media or tissue extracts will bind to functionally active PAI-1 coated on the microtiter plate (patent pending). Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. After appropriate washing steps, anti rat uPA primary antibody binds to the uPA. Excess antibody is washed away, and bound antibody, which is proportional to the original active uPA present in the sample, is then reacted with a secondary antibody conjugated to HRP. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared using dilutions of purified uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Gene Name: PLAU
Gene ID: 25619
Swiss-Prot/UniProt ID: P29598