The most powerful of the lysosomal proteases. It has a higher specific activity than cathepsin B and H in the degradation of a variety of physiological protein substrates. The level of cathepsin L is a strong predictor of relapse and survival following treatment of a primary breast tumor. Activity: Greater than 1 unit per mg of protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Z-Phe-Arg-AFC per minute at 25oC using 400 mM Na Acetate, pH 5.5 with 4 mM EDTA and 8 mM DTT as the activation buffer in the presence of Brij. Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. >95percent pure by SDS-PAGE.
Gene ID: 1514
Swiss-Prot/UniProt ID: P07711