Human factor IX is prepared from fresh frozen plasma by a combination of conventional procedures and immunoaffinity chromatography. This protein purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay. Human Factor IX, activated by either the Contact or Tissue Factor Pathway, is responsible for the activation of Factor X to Xa.
Gene ID: 2158
Swiss-Prot/UniProt ID: P00740