Human Factor XI will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XI and XIa will react with the antibody on the plate. After appropriate washing steps, peroxidase labeled polyclonal anti-human Factor XI primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of purified Factor XI and is measured along with the test samples. Color development is proportional to the concentration of Factor XI in the samples.
The Human Factor XI standard provided in this kit is now calibrated against the WHO International Standard for Human Plasma Factor XI (NIBSC Code 04/102).
Gene Name: F11
Gene ID: 2160
Swiss-Prot/UniProt ID: P03951