Factor Xa-beta is prepared by autoproteolysis of purified Factor Xa in the presence of calcium and phospholipid followed by chromatography over immobilized benzamidine. Cleavage occurs at the Arg290-Gly291 peptide bond. Purity is determined by SDS-PAGE analysis and activity is measured in a Factor Xa clotting assay and/or chromogenic substrate assay.
Gene Name: F10
Gene ID: 2159
Swiss-Prot/UniProt ID: P00742