P1'-NBD PAI-1 was created by mutagenesis of the methionine residue (Met347) at the P1-P1' scissile bond to cysteine. This then provides a free thiol group for NBD labeling, a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P1'-NBD PAI-1 is quenched upon cleavage of the reactive center loop by a target proteinase.
Gene Name: SERPINE1
Gene ID: 5054
Swiss-Prot/UniProt ID: P05121