Human wild type PAI-1 is produced as an active and latent form in E. Coli. The purification conditions are gentle and result in an active fraction >99percent pure and >98percent active as determined by titration with HMW tc-urokinase and SDS PAGE.
Gene Name: SERPINE1
Gene ID: 5054
Swiss-Prot/UniProt ID: P05121
Citations:
1. Adair, J.E. et al. (2009) J. Biol. Chem. 284:16922-16930.
2. Chai, J. et al. (2005) Cancer. Res. 65:10192-10198.
3. Jo, M. et al (2005) J. Biol. Chem. 280: 17449-17457.
4. Chu, J.W. et al. (2005) Diabetes and Vascular Disease Research 2: 37-41.
5. Lund, I.K. et al. (2008) J. Biol. Chem. 283: 32506-32515.
6. O’Connor, J.C et al. (2007) Mol. Cancer Res. 5: 585-603.
7. Moran, P. et al. (2006) J. Biol. Chem. 281: 30439-30446.
8. Chen, S. et al. (2008) Mol. Cancer Ther. 7: 1227-1236.
9. Li, S. et al. (2008) J. Biol. Chem. 283: 18147-18157.
10.Schar, C.R. et al. (2008) J. Biol. Chem. 283: 10297-10309.
11. Iafrati, M.D. (2005) Am. J. Physiol. Heart Circ. Physiol. 288: H1627-H1632.
12. Inagi, R. et al. (2007) Nephrol. Dial. Transplant 22:3311-3317.
13. Daniel Vial and Paula J. McKeown-Longo. (2008) J. Cell Sci. 121:1661-1670.
14. Erin H. Norris and Sidney Strickland (2007) PNAS 104: 13473-13478.
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