The sensitive measurement of total human uPA antigen is easily performed with this 96 well strip format ELISA kit. Human uPA present in plasma, serum, culture media or tissue extracts binds to the capture antibody coated on the micro titer plate. Free and complexed uPA will bind to the capture antibody. After appropriate washing steps, anti human uPA primary antibody binds to the uPA. Excess antibody is washed away, and bound antibody, which is proportional to the original total uPA present in the sample, is then reacted with a secondary antibody conjugated to HRP. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared using dilutions of purified uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Gene Name: PLAU
Gene ID: 5328
Swiss-Prot/UniProt ID: P00749