Mouse sc-tPA is reacted with an excess of mouse PAI-1 stable mutant at physiological pH. The resulting 1:1 molar complex is purified from the remaining PAI-1 by affinity chromatography with immobilized antibodies to mouse tPA.
Gene Name: SERPINE1
Gene ID: 18787, 18792
Swiss-Prot/UniProt ID: P22777, P06869