The sensitive measurement of total mouse PAI-1 antigen is easily performed with this 96 well strip format ELISA kit. Mouse PAI-1 binds capture antibody coated onto a micro titer plate. Free, latent and complexed mouse PAI-1 will bind to the capture antibody. Polyclonal rabbit anti-mouse PAI-1 primary antibody binds to the captured PAI-1. Excess antibody is washed away, and bound polyclonal antibody is then reacted with the secondary antibody. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Cross-reactivity: 2percent Porcine PAI, 1.7percent Human CPAI 0.5percent, Rabbit PAI, 0.3percent Rat PAI.

Negative control: PAI-1 genetically deficient mouse plasma
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Mouse PAI-1 Antigen Capture Plate, Secondary Antibody
Gene Name: SERPINE1
Gene ID: 18787
Swiss-Prot/UniProt ID: P22777


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