This wash buffer is ideal for washing ELISA plates between reagent addition steps. It is identical to the wash buffer concentrate supplied in our ELISA kits. Dilute concentrated wash buffer 1:10 with deionized water and mix well before use. May be applied with multichannel pipetter or by automatic plate washer. For best results, wash each well three times by adding 300 ul and aspirating completely. Store at 4C and bring to room temperature before use.