Acidified Citrate Anticoagulant

The following anticoagulants are recommended for PAI-1, tPA and uPA activity ELISA kits.

Stabilyte sodium citrate (pH 4.3)
Dissolve 66.2 g sodium citrate dihydrate in 450 ml distilled water. Adjust the pH to 4.3 then adjust volume to 500 ml with water. Sterilize by filtration through 0.22 um filters. Store up to 2 weeks at 4C.

Acidified sodium citrate (pH 5.2)
Dissolve 37.3 g sodium citrate and 8 g citric acid in 450 ml distilled water. Adjust the pH to 5.2 with NaOH, then adjust volume to 500 ml with water. Sterilize by filtration through 0.22 um filters. Store up to 2 weeks at 4C.

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