Fibrin – plate coating

1. Dilute fibrinogen to 1.75 uM in 0.05 M Na Phosphate, 0.15 M NaCl, pH 7.4 (PBS).
2. Add 80 ul fibrinogen to each well.
3. Dilute thrombin to 7.5 units/ml in PBS.
4. Add 20 ul thrombin to each well.
5. Cover lightly with kimwipe and incubate at room temperature overnight. The fluid will evaporate, leaving the fibrin clot stuck to the plate.
6. Wash wells three times with PBS.

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